Click chemistry and oligonucleotides conjugation

Over the past years we have developed the methodologies, and therefore have acquired a strong expertise, in chemoselective ligation that are crucial for designing DNA conjugates and chiefly DNA based biomolecular systems. More than 20 years ago, we pioneered the use of the oxime bond formation for DNA conjugation (Tetrahedron Lett. 1997). We investigated the oxime ligation for conjugation of oligonucleotides with a large variety of reporters such as peptides, carbohydrates, metal complexes as well as for attachment of ODN onto solid support. For the development of the TASQ concept  we have investigated the compatibility of oxime bond ligation with other click chemistry strategies (ChemistryOpen 2015) and successfully achieved the synthesis of several topological mimics of DNA structures of high biological interest (Chem. Eur. J. 2017).

More recently, in collaboration with Dr Samir Messaoudi (Biocis University Paris Orsay), we have developed an efficient Palladium-mediated Buchwald–Hartwig–Migita cross-coupling for the labelling of DNA via thio-glycoconjugation (Chem. Eur. J. 2018).