THOMAS LAVERGNE
Chargé de recherche (CNRS)
Affiliation
Centre national de la recherche scientifique
Équipe de recherche
DCM-I2BM
Domaines de recherche
Bioorganic Chemistry. Chemical Biology. Chemoselective Ligations. Molecular Recognition. Nucleic Acid Conjugates. Scaffold Assembly. DNA and RNA synthesis and modification. G-quadruplexes. In Vitro Selection. Aptamer. Combinatorial Chemistry.
Disciplines scientifiques
Chimie et vivant
Habilitation à diriger des recherches
Perenon, M., Bonnet H., Lavergne T., Dejeu J., & Defrancq E.
(2020). Surface plasmon resonance study of the interaction of N-methyl mesoporphyrin IX with G-quadruplex DNA.
Phys. Chem. Chem. Phys.. 22, 4158-4164.
Devaux, A., Bonnat L., Lavergne T., & Defrancq E.
(2020). Access to a stabilized i-motif DNA structure through four successive ligation reactions on a cyclopeptide scaffold.
Org. Biomol. Chem.. 18, 6394-6406.
Bonnat, L., Dautriche M., Saidi T., Revol-Cavalier J., Dejeu J., Defrancq E., et al.
(2019). Scaffold stabilization of a G-triplex and study of its interactions with G-quadruplex targeting ligands.
Org. Biomol. Chem.. 17, 8726-8736.
Bonnat, L., Dautriche M., Saidi T., Revol-Cavalier J., Dejeu J., Defrancq E., et al.
(2019). Scaffold stabilization of a G-triplex and study of its interactions with G-quadruplex targeting ligands..
Org. Biomol. Chem.. 17, 8726–8736.
Prado, E., Bonnat L., Bonnet H., Lavergne T., Van der Heyden A., Pratviel G., et al.
(2018). Influence of the SPR Experimental Conditions on the G-Quadruplex DNA Recognition by Porphyrin Derivatives..
Langmuir. 34, 13057–13064.
Piraux, G., Bar L., Abraham M., Lavergne T., Jamet H., Dejeu J., et al.
(2017). New Ruthenium-Based Probes for Selective G-Quadruplex Targeting..
Chem. - A Eur. J.. 23, 11872–11880.
Bonnat, L., Bar L., Gennaro B., Bonnet H., Jarjayes O., Thomas F., et al.
(2017). Template-mediated stabilization of a DNA G-quadruplex formed in the HIV-1 promoter and comparative binding studies..
Chem. - A Eur. J.. 23, 5602–5613.
Zhang, Y., Lamb B. M., Feldman A. W., Zhou A. Xiaozhou, Lavergne T., Li L., et al.
(2017). A semisynthetic organism engineered for the stable expansion of the genetic alphabet..
Proc. Natl. Acad. Sci. U. S. A.. 114, 1317–1322.
Lecarme, L., Prado E., De Rache A., Nicolau-Travers M-L., Gellon G., Dejeu J., et al.
(2016). Efficient Inhibition of Telomerase by Nickel-Salophen Complexes..
ChemMedChem. 11, 1133–1136.
Dejeu, J., Lavergne T., Nora J. Della, Defrancq E., & Pratviel G..
(2016). Binding of metalloporphyrins to G-quadruplex DNA: The role of the central metal..
Inorganica Chim. Acta. 452, 98–103.
Bonnat, L., Dejeu J., Bonnet H., Gennaro B., Jarjayes O., Thomas F., et al.
(2016). Templated Formation of Discrete RNA and DNA:RNA Hybrid G-Quadruplexes and Their Interactions with Targeting Ligands..
Chem. - A Eur. J.. 22, 3139–3147.
Lavergne, T., Lamichhane R.., Malyshev D.. A., Li Z.., Li L.., Sperling E.., et al.
(2016). FRET Characterization of Complex Conformational Changes in a Large 16S Ribosomal RNA Fragment Site-Specifically Labeled Using Unnatural Base Pairs.
Acs Chemical Biology. 11, 1347-53.
Bonnet, R., Lavergne T., Gennaro B., Spinelli N., & Defrancq E.
(2015). Construction of anti-parallel G-quadruplexes through sequential templated click..
Chem. Commun. (Cambridge, United Kingdom). 51, 4850–4853.
Stefan, L., Lavergne T., Spinelli N., Defrancq E., & Monchaud D..
(2014). Surface-immobilized DNAzyme-type biocatalysis..
Nanoscale. 6, 2693–2701.
Li, L.., Degardin M., Lavergne T., Malyshev D.. A., Dhami K.., Ordoukhanian P.., et al.
(2014). Natural-like replication of an unnatural base pair for the expansion of the genetic alphabet and biotechnology applications.
Journal of the American Chemical Society. 136, 826-9.
Malyshev, D.. A., Dhami K.., Lavergne T., Chen T.., Dai N.., Foster J.. M., et al.
(2014). A semi-synthetic organism with an expanded genetic alphabet.
Nature. 509, 385-8.
Li, Z.., Lavergne T., Malyshev D.. A., Zimmermann J.., Adhikary R.., Dhami K.., et al.
(2013). Site-specifically arraying small molecules or proteins on DNA using an expanded genetic alphabet.
Chemistry. 19, 14205-14209.
Baraguey, C.., Lescrinier E.., Lavergne T., Debart F.., Herdewijn P.., & Vasseur J.. J.
(2013). The biolabile 2'-O-pivaloyloxymethyl modification in an RNA helix: an NMR solution structure.
Organic & Biomolecular Chemistry. 11, 2638-47.
Betz, K.., Malyshev D.. A., Lavergne T., Welte W.., Diederichs K.., Romesberg F.. E., et al.
(2013). Structural insights into DNA replication without hydrogen bonds.
Journal of the American Chemical Society. 135, 18637-43.
Lavergne, T., Degardin M., Malyshev D.. A., Quach H.. T., Dhami K.., Ordoukhanian P.., et al.
(2013). Expanding the scope of replicable unnatural DNA: stepwise optimization of a predominantly hydrophobic base pair.
Journal of the American Chemical Society. 135, 5408-19.
Lavergne, T., Malyshev D.. A., & Romesberg F.. E.
(2012). Major groove substituents and polymerase recognition of a class of predominantly hydrophobic unnatural base pairs.
Chemistry. 18, 1231-9.
Malyshev, D.. A., Dhami K.., Quach H.. T., Lavergne T., Ordoukhanian P.., Torkamani A.., et al.
(2012). Efficient and sequence-independent replication of DNA containing a third base pair establishes a functional six-letter genetic alphabet.
Proc Natl Acad Sci U S A. 109, 12005-10.
Betz, K.., Malyshev D.. A., Lavergne T., Welte W.., Diederichs K.., Dwyer T.. J., et al.
(2012). KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry.
Nature Chemical Biology. 8, 612-4.
Seo, Y.. J., Malyshev D.. A., Lavergne T., Ordoukhanian P.., & Romesberg F.. E.
(2011). Site-specific labeling of DNA and RNA using an efficiently replicated and transcribed class of unnatural base pairs.
Journal of the American Chemical Society. 133, 19878-88.
Lavergne, T., Baraguey C.., Dupouy C.., Parey N.., Wuensche W.., Sczakiel G.., et al.
(2011). Synthesis and preliminary evaluation of pro-RNA 2'-O-masked with biolabile pivaloyloxymethyl groups in an RNA interference assay.
Journal of Organic Chemistry. 76, 5719-31.
Zlatev, I.., Lavergne T., Debart F.., Vasseur J.. J., Manoharan M.., & Morvan F..
(2010). Efficient solid-phase chemical synthesis of 5'-triphosphates of DNA, RNA, and their analogues.
Organic Letters. 12, 2190-3.
Lavergne, T., Janin M.., Dupouy C.., Vasseur J.. J., & Debart F..
(2010). Chemical synthesis of RNA with base-labile 2'-o-(pivaloyloxymethyl)-protected ribonucleoside phosphoramidites.
Curr Protoc Nucleic Acid Chem. Chapter 3, Unit3 19.
Martin, A.. R., Lavergne T., Vasseur J.. J., & Debart F..
(2009). Assessment of new 2'-O-acetalester protecting groups for regular RNA synthesis and original 2'-modified proRNA.
Bioorganic & Medicinal Chemistry Letters. 19, 4046-9.
Lavergne, T., Bertrand J.. R., Vasseur J.. J., & Debart F..
(2008). A base-labile group for 2'-OH protection of ribonucleosides: a major challenge for RNA synthesis.
Chemistry. 14, 9135-8.
Scientific Career
2017: HDR, Université Grenoble Alpes
Since 2013 : CNRS Researcher at DCM-I2BM, Université Grenoble Alpes, France
2013 : Post-Doc at DCM-I2BM, Université Grenoble Alpes, France
2010-2012 : Post-Doc at The Scripps Research Institute, California, USA
2006-2009 : PhD in Chemistry at IBMM, Montpellier, France
Thomas Lavergne is a CNRS researcher (section 16) since 2013.
After a master degree, at University of Montpellier 2, where he primarily studied the chemistry and biology of different classes of biomolecules, his Ph.D. research, under the supervision of Dr. Françoise Debart at IBMM-Montpellier (2006-2009), focused on nucleic acid chemistry. He primarily developed a new strategy for the chemical synthesis of RNA molecules and a methodology to assemble modified siRNA for diagnostic and therapeutic purposes.
In 2010, he joined the group of Prof. Floyd Romesbeg at the Scripps Research Institute (La Jolla, CA, USA). During his three years of post-doctoral experience, he shared his time between the multi-step synthesis of unnatural nucleosides and nucleotides for the expansion of the genetic alphabet, the in vitro and in vivo evaluation and structural characterization of such unnatural bases pairs and the site specific bioconjugation of the resulting unnatural DNA and RNA for biophysical studies and applications in nanotechnology.
At the present time, his research program focuses on the design, engineering and application of nucleic acid foldamers mimicking DNA and RNA secondary structures such as G-quadruplexes and i-motif. Those molecular objects are curently exploited, in collaboration, for the identification of selective binders (synthetic ligands or in vivo protein partners) and as catalysts and aptamers.
He is also engaged in the developement of novel classes of nucleic acid binders through combinatorial approaches.
This research is mainly established on bioorganic chemistry, nucleic acid chemistry (nucleoside, nucleotide, DNA and RNA), peptide chemistry, supramolecular chemistry, combinatorial chemistry and molecular biology, with the synthesis and manipulation of DNA and RNA conjugates, by chemical and enzymatic means.
Scientific Production
- Full publication list : Orcid: 0000-0002-0029-0015, ResearcherID: N-8896-2013- 26 publications including, 1 Nature, 1 Nat. Chem. Biol., 2 Proc. Natl. Acad. Sci. USA, 4 J. Am. Chem. Soc., 5 Chem. Eur. J., 1 Nanoscale, 1 Chem. Comm, etc.
- 2 licensed patents, 1 book chapter and 6 abstracts and proceedings
- >15 oral communications in international conferences and seminars (supported by fellowships from : iba-lifesciences, 5ECCLS and ACS Division of Biological Chemistry)
- Synthorx consultant since 2013
- Ganse-Liesel Award for Young Scientists 2014
Contact
Phone: +33 (0) 4 56 52 08 36
Fax: +33 (0) 4 56 52 08 05
E-mail: thomas.lavergne@univ-grenoble-alpes.fr
Last update: Jan-2018
TRAVAUX MAJEURS
- A base-labile group for 2'-OH protection of ribonucleosides: a major challenge for RNA synthesis
- A semi-synthetic organism with an expanded genetic alphabet
- Expanding the scope of replicable unnatural DNA: stepwise optimization of a predominantly hydrophobic base pair
- {Template-mediated stabilization of a DNA G-quadruplex formed in the HIV-1 promoter and comparative binding studies.}
- {Templated Formation of Discrete RNA and DNA:RNA Hybrid G-Quadruplexes and Their Interactions with Targeting Ligands.}