Using Native Chemical Ligation for Site-specific Synthesis of Hetero-bis-lanthanide Peptide Conjugates: Application to Ratiometric Visible or Near-infrared Detection of Zn2+
The interest for ratiometric luminescent probes that detect and quantify a specific analyte is growing. Due to their special luminescence properties, lanthanide(III) cations offer attractive opportunities for the design of dual-color ratiometric probes. Here, we describe the design principle of hetero-bis-lanthanide peptide conjugates using native chemical ligation for a perfect control of the localization of each lanthanide cation within the molecule. Two zinc-responsive probes, r-LZF1Tb|Cs124|Eu and r-LZF1Eu|Cs124|Tb are described on the basis of a zinc finger peptide and two DOTA complexes of terbium and europium. Both display dual-color ratiometric emission responding to the presence of Zn2+. Using a screening approach, anthracene was identified for the sensitization of the luminescence of two near infrared-emitting lanthanides, Yb3+ and Nd3+. Thus, two novel zinc-responsive hetero-bis-lanthanide probes, r-LZF3Yb|Anthra|Nd and r-LZF3Nd|Anthra|Yb were assembled, the former offering a neat ratiometric response to Zn2+ with emission in the near-infrared around 1000 nm, which is unprecedented.
Références
- Titre
- Using Native Chemical Ligation for Site-specific Synthesis of Hetero-bis-lanthanide Peptide Conjugates: Application to Ratiometric Visible or Near-infrared Detection of Zn2+
- Type de publication
- Article de revue
- Année de publication
- 2020
- Auteurs
- Cepeda, Celine, Raibaut Laurent, Fremy Guillaume, Eliseeva Svetlana V., Romieu Anthony, Pécaut Jacques, Boturyn Didier, Petoud Stephane, and Sénèque Olivier
- Revue
- Chemistry – A European Journal
- Volume
- 26
- Pagination
- 13476-13483
- Date de publication
- 07/2020
Soumis le 1 juillet 2020