Département de chimie moléculaire

The authors have developed a novel choline biosensor on the basis of immobilization of choline oxidase (ChOx) by the attractive materials layered double hydroxides (LDHs). Amperometric detection of choline was evaluated by holding the modified electrode at 0.5 V (vs. SCE). Due to the special properties of LDHs ([Zn3-Al-Cl]), such as chem. inertness, high porosity, and swelling property, the [Zn3-Al-Cl]/ChOx modified electrode exhibited an enhanced anal. performance. The biosensor provided a linear response to choline over a concn. range from 3.7 × 10-6 to 6.3 × 10-4 M with a low detection limit of 3 × 10-7 M based on S/N = 3. The apparent Michaelis-Menten const. is 1.38 mM. In addn., the interaction between ChOx and LDHs has also been studied using FTIR spectroscopy. [on SciFinder(R)]