Towards a Versatile Photoreactive Platform for Biosensing Applications

An original interfacial hybrid cross-linker molecule combined electrochemical and photochemical properties by substitution of two functional groups, pyrene, and diazirine. The first group enables anchoring by strong $π$-stacking interaction or by electropolymerization onto multi-walled carbon nanotubes (MWCNT). The other photoreactive group allows the covalent link with biomolecules under long-wavelength UV illumination. Diazirine was chosen due to its ability to photogenerate high reactive radicals under UV irradiation. The photoreactivity of these immobilized nanostructured conductive surfaces was tested towards the covalent attachment of tyrosinase which is well known to oxidize a large range of phenolic compounds and its yield and availability was evaluated by amperometric measurements of catechol by using molecular dioxygen. The architecture exhibiting the best analytical characteristics obtained for catechol was then chosen to detect dopamine.

Références

Titre
Towards a Versatile Photoreactive Platform for Biosensing Applications
Type de publication
Article de revue
Année de publication
2017
Revue
Journal of Analysis and Testing
Volume
1
Pagination
16
Date de publication
Jun
ISSN
2509-4696
Soumis le 18 mai 2018