Templated Formation of Discrete RNA and DNA:RNA Hybrid G-Quadruplexes and Their Interactions with Targeting Ligands.
G-rich RNA and DNA oligonucleotides derived from the human telomeric sequence were assembled onto addressable cyclopeptide platforms through oxime ligations and copper-catalyzed azide-alkyne cycloaddn. (CuAAc) reactions. The resulting conjugates were able to fold into highly stable RNA and DNA:RNA hybrid G-quadruplex (G4) architectures as demonstrated by UV, CD (CD), and NMR spectroscopic anal. Whereas rationally designed parallel RNA and DNA:RNA hybrid G4 topologies could be obtained, we could not force the formation of an antiparallel RNA G4 structure, thus supporting the idea that this topol. is strongly disfavored. The binding affinities of four representative G4 ligands toward the discrete RNA and DNA:RNA hybrid G4 topologies were compared to the one obtained with the corresponding DNA G4 structure. Surface plasmon resonance (SPR) binding anal. suggests that the accessibility to G4 recognition elements is different among the three structures and supports the idea that G4 ligands might be shaped to achieve structure selectivity in a biol. context. [on SciFinder(R)]
Références
- Titre
- Templated Formation of Discrete RNA and DNA:RNA Hybrid G-Quadruplexes and Their Interactions with Targeting Ligands.
- Type de publication
- Article de revue
- Année de publication
- 2016
- Auteurs
- Bonnat, Laureen, Dejeu Jérôme, Bonnet Hugues, Gennaro Béatrice, Jarjayes Olivier, Thomas Fabrice, Lavergne Thomas, and Defrancq Eric
- Revue
- Chem. - A Eur. J.
- Volume
- 22
- Pagination
- 3139–3147
- ISSN
- 0947-6539
Soumis le 12 avril 2018