A synthetic redox biofilm made from metalloprotein-prion domain chimera nanowires.

Engineering bioelectronic components and set-ups that mimic natural systems is extremely challenging. Here we report the design of a protein-only redox film inspired by the architecture of bacterial electroactive biofilms. The nanowire scaffold is formed using a chimeric protein that results from the attachment of a prion domain to a rubredoxin (Rd) that acts as an electron carrier. The prion domain self-assembles into stable fibers and provides a suitable arrangement of redox metal centers in Rd to permit electron transport. This results in highly organized films, able to transport electrons over several micrometres through a network of bionanowires. We demonstrate that our bionanowires can be used as electron-transfer mediators to build a bioelectrode for the electrocatalytic oxygen redn. by laccase. This approach opens opportunities for the engineering of protein-only electron mediators (with tunable redox potentials and optimized interactions with enzymes) and applications in the field of protein-only bioelectrodes. [on SciFinder(R)]

Références

Titre
A synthetic redox biofilm made from metalloprotein-prion domain chimera nanowires.
Type de publication
Article de revue
Année de publication
2017
Revue
Nat. Chem.
Volume
9
Pagination
157–163
ISSN
1755-4330
Soumis le 12 avril 2018