The study of multivalent carbohydrate-protein interactions by bio-layer interferometry.
The study of complex multivalent carbohydrate-protein interactions remains highly complicated and sometimes rendered impossible due to aggregation problems. In this study, we demonstrate that bio-layer interferometry is an excellent complementary method to std. techniques such as SPR and ITC. Using tetra- and hexadecavalent GalNAc glycoconjugates and Helix pomatia agglutinin (HPA) as a model lectin, we were able to measure reliable kinetic and thermodn. parameters of multivalent interactions going from the micro to the nanomolar range. [on SciFinder(R)]
Références
- Titre
- The study of multivalent carbohydrate-protein interactions by bio-layer interferometry.
- Type de publication
- Article de revue
- Année de publication
- 2018
- Auteurs
- Laigre, Eugenie, Goyard David, Tiertant Claire, Dejeu Jérôme, and Renaudet Olivier
- Revue
- Org. Biomol. Chem.
- Volume
- 16
- Pagination
- 8899–8903
- ISSN
- 1477-0520
Soumis le 5 avril 2019