Specificity of light-induced covalent adduct formation between RuII oligonucleotide conjugates and target sequences for gene silencing applications.

Under irradn., Ru-TAP (TAP = 1,4,5,8-tetraazaphenanthrene) complexes tethered to oligonucleotides (Ru-ODN) hybridized to their complementary target sequences produce irreversible adducts with guanine (G) bases. The interesting feature is that the G bases that react in the double strand (DS) topol. belong exclusively to the complementary strand; thus, no G base of the Ru-ODN strand participates in the prodn. of these adducts. This highly specific photoreaction in the DS topol. is demonstrated by gel electrophoresis analyses of different duplex sequences in which the positions of the G bases are varied. Atomistic mol. modeling simulations indicate that this specificity is due to important steric constraints for the intrastrand adduct formation in the duplex. Luminescence lifetime measurements furnish more detailed information on these different duplex systems. [on SciFinder(R)]

Références

Titre
Specificity of light-induced covalent adduct formation between RuII oligonucleotide conjugates and target sequences for gene silencing applications.
Type de publication
Article de revue
Année de publication
2014
Revue
Eur. J. Inorg. Chem.
Volume
2014
Pagination
3016–3022
ISSN
1434-1948
Soumis le 12 avril 2018