The Solution Structure of the ADAR2 dsRBM-RNA Complex Reveals a Sequence-Specific Readout of the Minor Groove.

Sequence-dependent recognition of dsDNA-binding proteins is well understood, yet sequence-specific recognition of dsRNA by proteins remains largely unknown, despite their importance in RNA maturation pathways. Adenosine deaminases that act on RNA (ADARs) recode genomic information by the site-selective deamination of adenosine. Here, we report the soln. structure of the ADAR2 double-stranded RNA-binding motifs (dsRBMs) bound to a stem-loop pre-mRNA encoding the R/G editing site of GluR-2. The structure provides a mol. basis for how dsRBMs recognize the shape, and also more surprisingly, the sequence of the dsRNA. The unexpected direct readout of the RNA primary sequence by dsRBMs is achieved via the minor groove of the dsRNA and this recognition is crit. for both editing and binding affinity at the R/G site of GluR-2. More generally, our findings suggest a soln. to the sequence-specific paradox faced by many dsRBM-contg. proteins that are involved in post-transcriptional regulation of gene expression. [on SciFinder(R)]

Références

Titre
The Solution Structure of the ADAR2 dsRBM-RNA Complex Reveals a Sequence-Specific Readout of the Minor Groove.
Type de publication
Article de revue
Année de publication
2010
Revue
Cell (Cambridge, MA, United States)
Volume
143
Pagination
225–237
ISSN
0092-8674
Soumis le 12 avril 2018