Département de chimie moléculaire

Based on the solid-state electrochemiluminescence (ECL) of an electrogenerated polypyrrole tagged with tris(2,2'-bipyridyl)ruthenium(II) complex ( denoted as polypyr-Ru), a new ethanol biosensor was fabricated by casting the composite of alc. dehydrogenase (ADH) and laponite colloids onto the polypyr-Ru /Pt electrode. The two layer structure keeps the good robustness of the biosensor due to the electrostatic interaction between the neg. charged biocomposite coating and the pos. charged polypyr-Ru. The morphol. of the laponite-ADH composite coating is characterized by SEM. The electrochem. and ECL properties of the modified electrodes are investigated by cyclic voltammetry. The parameters concerning the ECL biosensor such as the amt. of enzyme usage, the appropriate pH are optimized. The resulted ECL biosensor shows a good performance to detect ethanol. [on SciFinder(R)]