Sensitive and selective xanthine amperometric sensors based on calcium carbonate nanoparticles.

Using calcium carbonate nanoparticles as enzyme immobilization matrix, the developments of xanthine biosensor were achieved by both electrooxidn. and electroredn. of the enzymic-generated hydrogen peroxide based on xanthine oxidase (XnOx) and horseradish peroxidase (HRP). Amperometric detection of xanthine was evaluated by holding the modified electrode at 0.55 and -0.05 V (vs. SCE), for XnOx/Nano-CaCO3 and XnOx/HRP/Nano-CaCO3, resp. The linear dynamic ranges of anodic and cathodic detections of xanthine were between 2 × 10-6 to 2.5 × 10-4 M and 4 × 10-7 to 5 × 10-5 M, resp. The detection limits were detd. to be of 2 × 10-6 and 1 × 10-7 M with anodic and cathodic processes, resp. At lower working potential, XnOx/HRP/Nano-CaCO3/GCE bienzymic system exhibited excellent selectivity; the bienzyme electrode was inert towards ascorbic and uric acid present. Moreover, the permeability of enzyme/Nano-CaCO3 was evaluated by the use of rotating disk electrode voltammetry. [on SciFinder(R)]

Références

Titre
Sensitive and selective xanthine amperometric sensors based on calcium carbonate nanoparticles.
Type de publication
Article de revue
Année de publication
2009
Revue
Sensors Actuators, B Chem.
Volume
136
Pagination
510–515
ISSN
0925-4005
Soumis le 12 avril 2018