Département de chimie moléculaire

The feasibility of amperometric detection of rutin at a biosensor using polyphenol oxidase (PPO) as bioelement was investigated. The biosensor was fabricated by entrapment of PPO within a laponite clay film coated on an electrode surface. The amperometric detection of rutin was carried out at -0.1 V via the direct electrochem. redn. of the product of the enzymic reaction or its mediated redn. by a phenothiazine redox mediator (Azure B) intercalated within the clay matrix. The biosensor response was optimized with regard to pH, temp., applied potential (Eapp), and clay film thickness. The biosensor performance was discussed depending on the detection principle. [on SciFinder(R)]