Département de chimie moléculaire

A review. The concept of DNA biosensors is sustained by the need for rapid and highly sensitive anal. tools for genetic detection. Their implementation is based on three key steps: (i) immobilization of single-stranded oligonucleotide probes onto a substrate; (ii) hybridization and (iii) reading. These steps involve complementary knowledge in various disciplinary fields such as surface physics and chem., mol. electrochem., micro-technologies, optics, electronics and biochem. We present here, in a non-exhaustive way, the recent advances in the two steps of immobilization and detection that rely upon increasing integration of the no. of reading points or/and of the reading strategy. [on SciFinder(R)]