Quaternized chitosan as support for the assembly of gold nanoparticles and glucose oxidase: Physicochemical characterization of the platform and evaluation of its biocatalytic activity.
The authors report for the 1st time the use of quaternized chitosan (QCHI) for the immobilization of Au nanoparticles (NP) and glucose oxidase (GOD), the characterization of the resulting platform and its biocatalytic activity using glucose as substrate. The chem. substitution of chitosan has allowed the authors to work at physiol. pH to build up self-assembled layers of QCHI-NP as platform for the enzyme immobilization. The adsorption of GOD was analyzed by surface plasmon resonance (SPR) to compare the surface coverage of GOD in absence and presence of the QCHI-NP platform. The results obtained with cyclic voltammetry and scanning electrochem. microscopy (SECM) revealed that the adsorption of NP improves the cond. of the structure and its electrochem. reactivity, facilitating the oxidn. of the H2O2 produced by GOD. The electrodes modified with NP present higher amperometric response demonstrating the efficient transduction of the enzymic activity in this structure. [on SciFinder(R)]
Références
- Titre
- Quaternized chitosan as support for the assembly of gold nanoparticles and glucose oxidase: Physicochemical characterization of the platform and evaluation of its biocatalytic activity.
- Type de publication
- Article de revue
- Année de publication
- 2010
- Auteurs
- Bracamonte, Maria V., Bollo Soledad, Labbe Pierre, Rivas Gustavo A., and Ferreyra Nancy F.
- Revue
- Electrochim. Acta
- Volume
- 56
- Pagination
- 1316–1322
- ISSN
- 0013-4686
Soumis le 12 avril 2018