Département de chimie moléculaire

The attachment of biotin to nanotube modified electrodes by formation of $π$-stacking interactions using a biotinylated pyrene deriv. is reported. The specific anchoring of biol. macromols. via avidin bridges was investigated using biotinylated glucose oxidase (GOX-B) as model enzyme. Further studies focus the possibility of the electropolymn. of pyrene derivs. Four types of biotin-SWCNT modified electrodes were investigated: (1) deposits of biotin-pyrene functionalized nanotubes (B-SWCNTs); (2) deposits of regular nanotubes, functionalized by incubation in a biotin-pyrene soln.; (3) the latter configuration and subsequent electropolymn. of the adsorbed biotin-pyrene; and (4) deposits of regular nanotubes subsequently modified by electropolymn. of a pyrene-biotin monomer (2 mM). The nanotubes deposits were characterized by SEM imaging and the electropolymn. of the pyrene derivs. were investigated using cyclic voltammetry. These types of biotin-SWCNT modified electrodes were tested due to their capacity to immobilize biotinylated biomols. (GOX-B) via avidin bridges and their performances in glucose detection were examd. using amperometry. The best configuration (3) was obtained with SWCNT coatings modified by specific adsorption and electropolymn. of biotin-pyrene. A max. c.d. of 584 $μ$A cm-2 could be realized with a sensitivity of 37 mA M-1 cm-2 for a linear range between 5 $μ$M and 13 mM. [on SciFinder(R)]