Promotion of sugar-lectin recognition through the multiple sugar presentation offered by regioselectively addressable functionalized templates (RAFT): a QCM-D and SPR study.

The investigation of recognition events between carbohydrates and proteins, esp. the understanding of how spatial factors and binding avidity are correlated, remains a great interest for glycobiol. In this context we have investigated by nanogravimetry (QCM-D) and surface plasmon resonance (SPR), the kinetics and thermodn. of the interaction between Con A (Con A) and various neoglycopeptide ligands of low mol. wt. Regioselectively addressable functionalized templates (RAFT) have been used as scaffolds for the design of multivalent neoglycopeptides bearing thiol or biotin functions for their anchoring on transducer surfaces. Although these multivalent neoglycopeptide ligands cannot span multiple binding sites within the same Con A protein, they have increased activities relative to their monovalent counterpart. Our results emphasize that the multivalent RAFT ligands function by clustering several lectins, which leads to enhanced affinities. [on SciFinder(R)]

Références

Titre
Promotion of sugar-lectin recognition through the multiple sugar presentation offered by regioselectively addressable functionalized templates (RAFT): a QCM-D and SPR study.
Type de publication
Article de revue
Année de publication
2008
Revue
Org. Biomol. Chem.
Volume
6
Pagination
1114–1122
ISSN
1477-0520
Soumis le 12 avril 2018