Poly(methyl methacrylate) conductive fiber optic transducers as dual biosensor platforms.
Herein the development of an alternative optic-conductive fiber configuration applied for the construction of biosensing platforms. This new approach is based on applying the chem. polymn. of pyrrole onto the surface of polymethyl methacrylate (PMMA) fibers to create a polymer-a conductive surface, onto which an addnl. photoactive polypyrrole-benzophenone (PpyBz) film is electrochem. generated upon the fiber surface. Irradn. of the benzophenone groups embedded in the Ppy films with UV radiation (350 nm) formed active radicals that allowed the covalent attachment of the desired bioreceptors. Characterization of the amperometric biosensing matrix was accomplished by using a model Urease (Urs) through electrochem. impedance spectroscopy (EIS) and amperometry. Both techniques have shown a low charge transfer resistance (340 k$Ømega$) and a high sensitivity (12.3 $μ$A mM-1 cm-2). Thereafter, the construction of an optical biosensing matrix based on horseradish peroxidase (HRP) prodn. of photons was carried out. The high signal to noise (S/N) ratio (1600) indicated clearly that this approach can serve as a new platform to replace glass optical fibers based on biosensors. [on SciFinder(R)]
Références
- Titre
- Poly(methyl methacrylate) conductive fiber optic transducers as dual biosensor platforms.
- Type de publication
- Article de revue
- Année de publication
- 2009
- Auteurs
- Atias, Danit, Abu-Rabeah Khalil, Herrmann Sebastien, Frenkel Julia, Tavor Dorith, Cosnier Serge, and Marks Robert S.
- Revue
- Biosens. Bioelectron.
- Volume
- 24
- Pagination
- 3683–3687
- ISSN
- 0956-5663
Soumis le 12 avril 2018