Photo-Cross-Linking between Polymers Derivatized with Photoreactive Ruthenium-1,4,5,8-Tetraazaphenanthrene Complexes and Guanine-Containing Oligonucleotides.

We have shown previously that complexes contg. 1,4,5,8-tetraazaphenanthrene (TAP) ligands are able to form photoadducts with the guanine bases of DNA and oligonucleotides. In this work, we have exploited this specific photoreaction for carrying out photo-cross-linkings between guanine-contg. oligonucleotides (G-ODNs) and biodegradable polymers derivatized with the photoreactive Ru(II) compds. The aim in the future is to use these polymer conjugates as vectorizing agents of the metallic compds. inside the cells. Thus, photooxidizing Ru(II) complexes such as [Ru(TAP)3]2+ and [Ru(TAP)2phen]2+ (phen = 1,10-phenanthroline) have been derivatized by an oxyamine function to attach them, via an oxime ether linkage, to a sol. 6 or 80 kDa poly-[N-(2-hydroxyethyl)-L-glutamine] polymer that contains pendent aldehyde groups. It is demonstrated that the resulting Ru-labeled polymers exhibit photophys. properties and a photochem. that are comparable with those of the free, nonattached complexes. The photo-cross-linkings with the G-ODNs are clearly detected by gel electrophoresis with the 6 kDa Ru conjugates upon illumination. [on SciFinder(R)]

Références

Titre
Photo-Cross-Linking between Polymers Derivatized with Photoreactive Ruthenium-1,4,5,8-Tetraazaphenanthrene Complexes and Guanine-Containing Oligonucleotides.
Type de publication
Article de revue
Année de publication
2007
Revue
Biomacromolecules
Volume
8
Pagination
3503–3510
ISSN
1525-7797
Soumis le 12 avril 2018