Département de chimie moléculaire

We have shown previously that complexes contg. 1,4,5,8-tetraazaphenanthrene (TAP) ligands are able to form photoadducts with the guanine bases of DNA and oligonucleotides. In this work, we have exploited this specific photoreaction for carrying out photo-cross-linkings between guanine-contg. oligonucleotides (G-ODNs) and biodegradable polymers derivatized with the photoreactive Ru(II) compds. The aim in the future is to use these polymer conjugates as vectorizing agents of the metallic compds. inside the cells. Thus, photooxidizing Ru(II) complexes such as [Ru(TAP)3]2+ and [Ru(TAP)2phen]2+ (phen = 1,10-phenanthroline) have been derivatized by an oxyamine function to attach them, via an oxime ether linkage, to a sol. 6 or 80 kDa poly-[N-(2-hydroxyethyl)-L-glutamine] polymer that contains pendent aldehyde groups. It is demonstrated that the resulting Ru-labeled polymers exhibit photophys. properties and a photochem. that are comparable with those of the free, nonattached complexes. The photo-cross-linkings with the G-ODNs are clearly detected by gel electrophoresis with the 6 kDa Ru conjugates upon illumination. [on SciFinder(R)]