Département de chimie moléculaire

The 5'-phosphorylated oligonucleotides (5'-pONs) are currently synthesized using expensive and sensitive modified phosphoramidite reagents. In this work, a simple, cost-effective, efficient, and automated method is presented, based on the controlled oxidn. of the 5'-terminal alc. followed by a $\beta$-elimination reaction. The latter reaction leads to the removal of the terminal 5'-nucleoside and subsequent formation of the 5'-phosphate moiety. Thus, chem. phosphorylation of oligonucleotides (DNA or RNA) is achieved without using modified phosphoramidites. [on SciFinder(R)]