Parameters influencing the photo-induced electron transfer from tryptophan-containing peptides to a RuII complex: a systematic study.
Ruthenium(II) polyazaarom. complexes have gained interest in recent decades as biomol. tools, esp. in the development of new phototherapeutic agents. These light emissive Ru complexes based on $π$-deficient ligands were first designed to allow a photo-induced electron transfer (PET) with the guanine base in DNA since their 3MLCT state is highly photo-oxidizing. Later the field of research was extended to proteins with the highlighting of a PET process with the tryptophan residue. This paper reports the kinetics of the luminescence quenching of [Ru(TAP)2phen]2+ by several selected peptide sequences contg. at least one tryptophan residue. By using a peptide library we highlight the important parameters influencing the kinetics of the photo-electron transfer process, such as the net electrostatic charge and the no. of tryptophan residues. The best peptide candidates were selected to study the formation of photo-products by MALDI-ToF mass spectrometry. A high photoreactivity of the [Ru(TAP)2phen]2+ complex was obsd. and multiple photoadducts were characterized, among them inter-peptidic adducts as well as intra-peptidic adducts. [on SciFinder(R)]
Références
- Titre
- Parameters influencing the photo-induced electron transfer from tryptophan-containing peptides to a RuII complex: a systematic study.
- Type de publication
- Article de revue
- Année de publication
- 2015
- Auteurs
- Garnir, Kevin, Estalayo-Adrian Sandra, Lartia Remy, De Winter Julien, Defrancq Eric, Surin Mathieu, Lemaur Vincent, Gerbaux Pascal, and Moucheron Cecile.
- Revue
- Faraday Discuss.
- Volume
- 185
- Pagination
- 267–284
- ISSN
- 1359-6640
Soumis le 12 avril 2018