Parameters influencing the photo-induced electron transfer from tryptophan-containing peptides to a RuII complex: a systematic study.

Ruthenium(II) polyazaarom. complexes have gained interest in recent decades as biomol. tools, esp. in the development of new phototherapeutic agents. These light emissive Ru complexes based on $π$-deficient ligands were first designed to allow a photo-induced electron transfer (PET) with the guanine base in DNA since their 3MLCT state is highly photo-oxidizing. Later the field of research was extended to proteins with the highlighting of a PET process with the tryptophan residue. This paper reports the kinetics of the luminescence quenching of [Ru(TAP)2phen]2+ by several selected peptide sequences contg. at least one tryptophan residue. By using a peptide library we highlight the important parameters influencing the kinetics of the photo-electron transfer process, such as the net electrostatic charge and the no. of tryptophan residues. The best peptide candidates were selected to study the formation of photo-products by MALDI-ToF mass spectrometry. A high photoreactivity of the [Ru(TAP)2phen]2+ complex was obsd. and multiple photoadducts were characterized, among them inter-peptidic adducts as well as intra-peptidic adducts. [on SciFinder(R)]

Références

Titre
Parameters influencing the photo-induced electron transfer from tryptophan-containing peptides to a RuII complex: a systematic study.
Type de publication
Article de revue
Année de publication
2015
Revue
Faraday Discuss.
Volume
185
Pagination
267–284
ISSN
1359-6640
Soumis le 12 avril 2018