Oligonucleotide-selenide conjugate: Synthesis and its inducible sequence-specific alkylation of DNA.

Oligonucleotide-selenium conjugate was designed and synthesized and its sequence-specific crosslinking ability was investigated. The selenide derivs. can generate covalent interstrand crosslinking with its complementary strand through the formation of o-QM intermediate induced by periodate oxidn. A crosslinking reaction yield of up to 50{%} was obtained. Hydroxyl radical footprinting expt. revealed that the quinone appendage specifically alkylated the cytosine base extending the duplex formed between the conjugate and the target strand. [on SciFinder(R)]

Références

Titre
Oligonucleotide-selenide conjugate: Synthesis and its inducible sequence-specific alkylation of DNA.
Type de publication
Article de revue
Année de publication
2010
Revue
Bioorg. Med. Chem.
Volume
18
Pagination
4149–4153
ISSN
0968-0896
Soumis le 12 avril 2018