Non-covalent biofunctionalization of single-walled carbon nanotubes via biotin attachment by $π$-stacking interactions and pyrrole polymerization.

Single-walled carbon nanotubes were functionalized with biotin using either electropolymn. or formation of $π$-stacking interactions for the construction of biosensors. Thanks to the high affinity of the avidin-biotin interactions, a biotinylated glucose oxidase (B-GOX) as a biomol. model was immobilized on the biotinylated nanotubes. The influence of the biosensor configuration on their amperometric performances was investigated by changing the amt. of nanotubes and the nos. of avidin/B-GOX layers. By increasing the amt. of nanotube and avidin/B-GOX layers, both sensor setups show a perfect linear increase of immobilized enzymes reflecting a high reproducibility of the authors' systems. The highest sensitivities (up to 5.2 mA M-1 cm-2) and max. current densities (up to 55 $μ$A cm-2) were obtained using nanotube deposits modified by electrochem. coatings. Noncovalently functionalized biotin-nanotubes show a better permeability for the enzymically generated hydrogen peroxide. [on SciFinder(R)]

Références

Titre
Non-covalent biofunctionalization of single-walled carbon nanotubes via biotin attachment by $π$-stacking interactions and pyrrole polymerization.
Type de publication
Article de revue
Année de publication
2009
Revue
Anal. (Cambridge, United Kingdom)
Volume
134
Pagination
2412–2418
ISSN
0003-2654
Soumis le 12 avril 2018