Non-covalent biofunctionalization of single-walled carbon nanotubes via biotin attachment by $π$-stacking interactions and pyrrole polymerization.
Single-walled carbon nanotubes were functionalized with biotin using either electropolymn. or formation of $π$-stacking interactions for the construction of biosensors. Thanks to the high affinity of the avidin-biotin interactions, a biotinylated glucose oxidase (B-GOX) as a biomol. model was immobilized on the biotinylated nanotubes. The influence of the biosensor configuration on their amperometric performances was investigated by changing the amt. of nanotubes and the nos. of avidin/B-GOX layers. By increasing the amt. of nanotube and avidin/B-GOX layers, both sensor setups show a perfect linear increase of immobilized enzymes reflecting a high reproducibility of the authors' systems. The highest sensitivities (up to 5.2 mA M-1 cm-2) and max. current densities (up to 55 $μ$A cm-2) were obtained using nanotube deposits modified by electrochem. coatings. Noncovalently functionalized biotin-nanotubes show a better permeability for the enzymically generated hydrogen peroxide. [on SciFinder(R)]
Références
- Titre
- Non-covalent biofunctionalization of single-walled carbon nanotubes via biotin attachment by $π$-stacking interactions and pyrrole polymerization.
- Type de publication
- Article de revue
- Année de publication
- 2009
- Auteurs
- Haddad, R, Cosnier Serge, Maaref A, and Holzinger Michael
- Revue
- Anal. (Cambridge, United Kingdom)
- Volume
- 134
- Pagination
- 2412–2418
- ISSN
- 0003-2654
- Mots-clés
- pi stacking biotin noncovalent biofunctionalization single walled carbon nanotube pyrrole polymn biotin noncovalent biofunctionalization single walled carbon nanotube
- DOI
- 10.1039/b916774a
Soumis le 12 avril 2018