Magnetic Zirconium Hexacyanoferrate(II) Nanoparticle as Tracing Tag for Electrochemical DNA Assay.
Novel multifunctional magnetic zirconium hexacyanoferrate nanoparticles (ZrHCF MNPs) were prepd., which consisted of magnetic beads (MBs) inner core and zirconium hexacyanoferrate(II) (ZrHCF) outer shell. As an artificial peroxidase, the ZrHCF MNPs exhibited remarkable electrocatalytic properties in the redn. of H2O2 at 0.2 V vs. SCE. On the basis of the bonding interaction between Zr(IV) of the shell ZrHCF framework and phosphonate groups, the 5'-phosphorylated ssDNA probes with a consecutive stretch of guanines as a spacer could be incorporated in ZrHCF MNPs easily. Thus, DNA-grafted ZrHCF MNPs could be simply obtained by magnetic sepn. The prepd. nanoelectrocatalyst was further used as a signal nanoprobe for the ultrasensitive electrochem. DNA assay. Under optimal conditions, the proposed biosensor presents high sensitivity for detecting target DNA with a linear range from 1.0 fM to 1.0 nM and a low detection limit of 0.43 fM. Moreover, it exhibits good performance with excellent selectivity, high stability, and acceptable fabrication reproducibility. [on SciFinder(R)]
Références
- Titre
- Magnetic Zirconium Hexacyanoferrate(II) Nanoparticle as Tracing Tag for Electrochemical DNA Assay.
- Type de publication
- Article de revue
- Année de publication
- 2015
- Auteurs
- Zhang, Guang-Yao, Deng Sheng-Yuan, Cai Wen-Rong, Cosnier Serge, Zhang Xue-Ji, and Shan Dan.
- Revue
- Anal. Chem. (Washington, DC, United States)
- Volume
- 87
- Pagination
- 9093–9100
- ISSN
- 0003-2700
Soumis le 12 avril 2018