Label-free detection of cupric ions and histidine-tagged proteins using single poly(pyrrole)-NTA chelator conducting polymer nanotube chemoresistive sensor.

Novel chem. and biol. sensors based on a single poly(pyrrole)-NTA chelator nanotube for sensitive, selective, rapid and real-time detection of histidine-tagged protein and cupric ions are reported. NTA groups on the nanotube surface provided a simple mechanism for metal ion sensing via the high-affinity interaction between NTA and the subsequent detection of histidine-tagged protein through the coordination with metal chelated nanotube. Poly(pyrrole)-NTA chelator nanotubes of 190 nm outside diam., 35 nm wall thickness and 30 $μ$m long were synthesized by electrochem. polymn. of pyrrole-NTA inside a 200 nm diam. alumina template and assembled as a chemoresistive device by bottom-up contact geometry on a pair of parallel gold electrodes with a gap distance of 3 $μ$m. The chemoresistive sensors based on single poly(pyrrole)-NTA chelator nanotube exhibited detection as low as one-hundredth attomolar (0.6 ppt) cupric ions and 1 ng/mL of penta-histidine tagged syntaxin protein. [on SciFinder(R)]

Références

Titre
Label-free detection of cupric ions and histidine-tagged proteins using single poly(pyrrole)-NTA chelator conducting polymer nanotube chemoresistive sensor.
Type de publication
Article de revue
Année de publication
2009
Revue
Biosens. Bioelectron.
Volume
24
Pagination
1451–1455
ISSN
0956-5663
Soumis le 12 avril 2018