An L-Lactate Amperometric Enzyme Electrode Based on L-Lactate Oxidase Immobilized in a Laponite Gel on a Glassy Carbon Electrode. Application to Dairy Products and Red Wine.
A biosensor based on the immobilization of Lactate oxidase in laponite-organosilasesquioxane films on glassy carbon electrode for the quantification of L-lactate in wine and dairy products is presented. The bioelectrode showed a very high sensitivity (0.33±0.01) A cm-2 M-1 and a short time response (10 s) for less than 1 U of enzyme. No significant interferences, including ascorbic acid, were detected. For red wine, matrix effects assigned to polyphenols and anthocyanins were obsd., which were easily overcome by sample diln. Our L-lactate detns. were in good agreement with those of two std. methods. [on SciFinder(R)]
Références
- Titre
- An L-Lactate Amperometric Enzyme Electrode Based on L-Lactate Oxidase Immobilized in a Laponite Gel on a Glassy Carbon Electrode. Application to Dairy Products and Red Wine.
- Type de publication
- Article de revue
- Année de publication
- 2010
- Auteurs
- Zanini, Veronica Paz, de Mishima Beatriz Lopez, Labbe Pierre, and Solis Velia.
- Revue
- Electroanalysis
- Volume
- 22
- Pagination
- 946–954
- ISSN
- 1040-0397
Soumis le 12 avril 2018