KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry

Many candidate unnatural DNA base pairs have been developed, but some of the best-replicated pairs adopt intercalated structures in free DNA that are difficult to reconcile with known mechanisms of polymerase recognition. Here we present crystal structures of KlenTaq DNA polymerase at different stages of replication for one such pair, dNaM-d5SICS, and show that efficient replication results from the polymerase itself, inducing the required natural-like structure.

Références

Titre
KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry
Type de publication
Article de revue
Année de publication
2012
Revue
Nature Chemical Biology
Volume
8
Pagination
612-4
Soumis le 5 juillet 2018