KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry
Many candidate unnatural DNA base pairs have been developed, but some of the best-replicated pairs adopt intercalated structures in free DNA that are difficult to reconcile with known mechanisms of polymerase recognition. Here we present crystal structures of KlenTaq DNA polymerase at different stages of replication for one such pair, dNaM-d5SICS, and show that efficient replication results from the polymerase itself, inducing the required natural-like structure.
Références
- Titre
- KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry
- Type de publication
- Article de revue
- Année de publication
- 2012
- Auteurs
- Betz, K., Malyshev D. A., Lavergne Thomas, Welte W., Diederichs K., Dwyer T. J., Ordoukhanian P., Romesberg F. E., and Marx A.
- Revue
- Nature Chemical Biology
- Volume
- 8
- Pagination
- 612-4
Soumis le 5 juillet 2018