Département de chimie moléculaire

We present herein an effective and versatile method to fabricate a micro-patterned structure of conductive polymer, poly(pyrrole-benzophenone), on Indium Tin Oxide (ITO) glass chips for the subsequent photo-immobilization of various bioreceptor, antigens. Such methodologies are based on photolithog. of ITO pattern fabrication on non-conductive surfaces, glass slides, and on a photo-active electrogenerated polymer films. The photo-active polymer serves as a substrate platform for the photo-immobilization of the bioreceptor reagents used for subsequent immunoreactions. We were able to show the resoln. of electropolymn. on an ITO pattern as well as immobilization of more than one bioreceptor for the simultaneous detection of several analytes. The antigen microarrays were tested for sensitivity, specificity, and overall practicality for the simultaneous detection of analyte anti-Cholera Toxin B, anti-Hepatitis B virus surface and core protein antibodies. In addn. we used our pattern ITO-poly(pyrrole-benzophenone) micro-array for the detection of serum samples of Hepatitis B virus patients previously screened by a std. hospital detection method. [on SciFinder(R)]