Influence of the SPR Experimental Conditions on the G-Quadruplex DNA Recognition by Porphyrin Derivatives.

Surface plasmon resonance (SPR) is a powerful technique to study the interactions of ligands with analytes and therefore a no. of biosensor surfaces and injection methods have been developed so far. However, many exptl. parameters can affect the interactions and consequently the affinity measurements. In particular, the interactions of pos. charged analytes (often used for anionic nucleic acids targets) can be influenced by the sensing surfaces (e.g., neg. charged), leading to significant nonspecific interactions as well as regeneration problems. The aim of the present work is to study the effect of different parameters, including ionic strength, SPR biosensor (i.e., nature of the surfaces), and the injection method on the recognition of porphyrin G-quadruplex ligands. The injection method does not influence the affinity whereas the ionic strength and the nature of the surface impact the recognition properties of the porphyrin for the G-quadruplex DNA. Also self-assembled monolayer coating surface presents many advantages in comparison with carboxymethylated dextran surface for SPR studies of G-quadruplex DNA/ligand interactions: (1) the electrostatic interaction with charged analytes is less important, (2) its structure/compn. is less sensitive to the ionic concn. and less prone to unspecific adsorption, (3) it is easily homemade, and (4) the cost is ∼10 times cheaper. [on SciFinder(R)]

Références

Titre
Influence of the SPR Experimental Conditions on the G-Quadruplex DNA Recognition by Porphyrin Derivatives.
Type de publication
Article de revue
Année de publication
2018
Revue
Langmuir
Volume
34
Pagination
13057–13064
ISSN
0743-7463
Soumis le 5 avril 2019