Impedimetric immunosensor for the specific label free detection of ciprofloxacin antibiotic.

Impedance spectroscopy approaches combined with the immunosensor technol. were used for the detn. of trace amts. of ciprofloxacin antibiotic belonging to the fluoroquinolone family. The sensor electrode was based on the immobilization of anti-ciprofloxacin antibodies by chem. binding onto a poly(pyrrole-NHS) film electrogenerated on a solid gold substrate. The electrode surface was modified by electropolymn. of pyrrole-NHS, antibody grafting and ciprofloxacin immunoreaction. The sensitive steps of surface modification, cyclic voltammetry (CV), and at. force microscopy (AFM) imaging were used for electrode surface characterization. The immunoreaction of ciprofloxacin on the grafted anti-ciprofloxacin antibody directly triggers a signal via impedance spectroscopy measurements which allows the detection of extremely low concn. of 10 pg/mL ciprofloxacin. [on SciFinder(R)]

Références

Titre
Impedimetric immunosensor for the specific label free detection of ciprofloxacin antibiotic.
Type de publication
Article de revue
Année de publication
2007
Revue
Biosens. Bioelectron.
Volume
23
Pagination
549–555
ISSN
0956-5663
Soumis le 12 avril 2018