Impedimetric biosensor for cancer cell detection.

Electrochem. impedance spectroscopy was evaluated for the label free detection of MCF-7 cancer cell in which c-erbB-2 receptor is overexpressed on the cell surfaces. Anti-c-erbB-2, used as a specific antibody, was immobilized on electrogenerated polypyrrole-NHS on electrodes via covalent linking. The polymer formation, the grafting of the antibody, and the recognition event with the cancer cells using MCF-7 as a model cell line, were characterized by using cyclic voltammetry and fluorescence microscopy. The impedimetric sensor showed high sensitivity from 100 to 10 000 cell/mL without needing any labeling step and represents an efficient transduction method for cell selective detection. [on SciFinder(R)]

Références

Titre
Impedimetric biosensor for cancer cell detection.
Type de publication
Article de revue
Année de publication
2013
Revue
Electrochem. commun.
Volume
37
Pagination
36–39
ISSN
1388-2481
Soumis le 12 avril 2018