Département de chimie moléculaire

A novel and simple immobilization strategy for biotinylated biol. macromols. onto electropolymd. poly(pyrrole-nitrilotriacetic acid)(NTA)-Cu2+ films without avidin as connecting bridge is reported. After complexation of Cu2+ by the polymd. NTA chelator, biotinylated biomols. were immobilized by coordination of the biotin groups on the NTA-Cu2+ complex. The anchoring of biotinylated glucose oxidase was demonstrated by fluorescent characterization via FITC-labeled avidin and amperometric measurement of glucose. The resulting calibration curve led to a sensitivity and max. c.d. values of 0.6 mA mol-1 L cm-2 and 13.2 $μ$A cm-2, resp. Thus, biotinylated polyphenol oxidase was fixed leading to a catechol sensor with a sensitivity of 656 mA mol-1 L cm-2 and max. c.d. of 25.4 $μ$A cm-2. This system was also applied to the efficient immobilization of biotinylated DNA, illustrated by impedimetric detection of the formation of the DNA duplex. [on SciFinder(R)]