Département de chimie moléculaire

We report the efficient immobilization and orientation of laccase from Trametes versicolor on MWCNT electrodes using 1-pyrenebutyric acid adamantyl amide as a supramol. linker. We demonstrate the ability of adamantane to specifically interact with the hydrophobic cavity of laccase, while pyrene interacts with MWCNT sidewalls by $π$-$π$ interactions. Adamantane allows the oriented immobilization of laccases on MWCNT electrodes. Using an anthraquinone-modified pyrene deriv. for comparison, adamantane-modified MWCNTs achieve the stable immobilization and orientation of a higher no. of enzymes per surface units, as confirmed by electrochem., theor. calcns., and quartz crystal microbalance expts. Furthermore, the efficient direct electron transfer ensures bioelectrocatalytic oxygen redn. at high half-wave potential of 0.55 V vs SCE accompanied by no kinetic limitation by the heterogeneous electron transfer and max. current densities of 2.4 mA cm-2. [on SciFinder(R)]