Département de chimie moléculaire

A biosensor for amperometric detn. of nitrite was developed using cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans immobilized and elec. connected on a glassy carbon electrode by entrapment into redox active [ZnCr-AQS] layered double hydroxide contg. anthraquinone-2-sulfonate (AQS). The transduction step corresponded to the electro-enzymic redn. of nitrite by immobilized AQS mols. at -0.6 V. The biosensor showed a fast response to nitrite (5 s) with a linear range between 0.015 and 2.35 $μ$M, a sensitivity of 1.8 A M-1 cm-2 and a detection limit of 4 nM. The apparent Michaelis-Menten const. (KaMpp) was 7.5 $μ$M. [on SciFinder(R)]