Département de chimie moléculaire

An electrochem. highly sensitive aptasensor was developed based on electropolymd. poly(pyrrole-nitrilotriacetic) acid film and a new aptamer functionalized by a pentahistidine peptide for the quantification of bisphenol A. A surface coverage of antibisphenol A aptamer of 1.84 × 10-10 mol cm-2 was estd. from the electrochem. signal of the [RuIII(NH3)6]3+ complex bound by electrostatic interactions onto the aptamer-modified electrode. The binding of bisphenol A onto the polymer film was successfully characterized by electrochem. methods as square wave voltammetry and electrochem. impedance spectroscopy measurements. The designed label-free impedimetric aptasensor displayed a wide linear range from 10-11 to 10-6 mol L-1 with a sensitivity of 372 $Ømega$ per unit of log of concn. and an excellent specificity toward interfering agents such as 4,4'-dihydroxybiphenyl and bisphenol P. [on SciFinder(R)]