Highly DNA-Photoreactive Ruthenium 1,4,5,8-Tetraazaphenanthrene Complex Conjugated to the TAT Peptide: Efficient Vectorization inside HeLa Cells without Phototoxicity - The Importance of Cellular Distribution.

The photoreactive [Ru(TAP)2(phen)]2+ (TAP = 1,4,5,8-tetraazaphenanthrene; phen = 1,10-phenanthroline) complex tethered to the cell-penetrating peptide (CPP) TAT was studied in vitro and in cellulo. The tethering of the complex does not affect its behavior under blue-light irradn. in the presence of guanine-contg. oligodeoxyribonucleotides (ODNG). Thus, the luminescence is quenched in the presence of ODNG, and gel electrophoresis expts. showed the appearance of products corresponding to the irreversible attachment of the conjugate to ODNG upon illumination. The cellular uptake of the conjugate was examd. by flow cytometry, inductively coupled plasma mass spectrometry (ICP-MS), and confocal imaging microscopy. The [Ru(TAP)2(phen-TAT)] conjugate is readily taken up by HeLa cells and, despite these favorable factors, the cellular survival was 100 {%}, as measured by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. A possible origin of the inactivity of [Ru(TAP)2(phen-TAT)] under irradn. is proposed on the basis of the fluorescence-activated cell sorting (FACS), ICP-MS, and confocal microscopy results. [on SciFinder(R)]

Références

Titre
Highly DNA-Photoreactive Ruthenium 1,4,5,8-Tetraazaphenanthrene Complex Conjugated to the TAT Peptide: Efficient Vectorization inside HeLa Cells without Phototoxicity - The Importance of Cellular Distribution.
Type de publication
Article de revue
Année de publication
2016
Revue
Eur. J. Inorg. Chem.
Volume
2016
Pagination
2902–2911
ISSN
1434-1948
Soumis le 12 avril 2018