Département de chimie moléculaire

We describe herein the fabrication of bioelectrodes by compression of a mixt. of graphite particles, enzyme and redox mediator that leads to the immobilization and elec. connection of enzymes. Bioanodes were elaborated by combining graphite with glucose oxidase and ferrocene while biocathodes resulted from the assocn. of graphite, laccase and ABTS. The influence of the enzyme loading on the electrocatalytic properties of bioelectrodes for glucose oxidn. and oxygen redn. was examd. and an optimum enzyme loading was detd.: 5{%} (wt./wt.). The modulation of the mediator loading led to optimum compns. of 45 and 50{%} for ABTS and ferrocene, resp. A biofuel cell configuration was assembled and its performances were investigated as a function of pH providing, at pH 6, a max. power d. and an open circuit voltage of 207 $μ$W cm-2 and 450 mV, resp. [on SciFinder(R)]