Functional characterization of starch-degrading enzymes using quartz crystal microbalance with dissipation monitoring (QCM-D).
During the last few decades, starch-degrading enzymes have emerged as promising tools for transforming biomass. Nevertheless, their functional characterization, based essentially on colorimetric tests, is still fastidious and time-consuming. In order to overcome these hurdles, we have developed a test for real-time and label-free detection of starch-degrading enzymes using quartz crystal microbalance with dissipation monitoring (QCM-D). By starch-functionalization of surfaces, we were able to discriminate clearly enzymes with different hydrolysis patterns (endo vs. exo). The variation of the hydrolytic activity between the wild-type and a mutant of a cyclodextrin glucosyl transferases could also be monitored acoustically. The high sensitivity reached (down to 6.5 nkat/L) opens the prospect of using fewer enzyme quantities for functional tests and is a further advantage for this easily used and efficient tool for enzyme characterization. [on SciFinder(R)]
Références
- Titre
- Functional characterization of starch-degrading enzymes using quartz crystal microbalance with dissipation monitoring (QCM-D).
- Type de publication
- Article de revue
- Année de publication
- 2013
- Auteurs
- Bouchet-Spinelli, Aurelie, Guerente Liliane, Armand Sylvie, Lenouvel Francois, Labbe Pierre, and Fort Sebastien.
- Revue
- Sensors Actuators, B Chem.
- Volume
- 176
- Pagination
- 1038–1043
- ISSN
- 0925-4005
Soumis le 12 avril 2018