Département de chimie moléculaire

The fully solid-phase synthesis of chem. well-defined glycoclusters grafted to a topol. cyclodecapeptide template is described. The orthogonally-protected peptide backbone was first synthesized and cyclized on solid support using D-glutamic acid as first amino acid linked to the resin. After successive regioselective deprotection steps, biotin groups were coupled to the lower addressable domains of the scaffold, then carbohydrates-binding ligands were assembled as cluster on the upper domain using a chemoselective oxime-based strategy. This provides multitopic labeled glycopeptides which can be easily immobilized to streptavidin-coated surfaces for studying carbohydrate-protein interactions in glycomic researches. [on SciFinder(R)]