Département de chimie moléculaire

This study shows that a cationic esterase mimic, which is responsible for the catalytic hydrolysis of a neg. charged fluorogenic ester, displays different inhibition behaviors in the presence of similar anionic metabolites in water and at physiol. pH. On the contrary of the other neg. charged inhibitors tested, ADP strongly slowed down the formation of the fluorescent product by virtue of cooperative inhibition, hence allowing its spectroscopic or visual detection. The authors' sensor or its derivs. may find future use in biol. applications, such as protein kinase activity detection. [on SciFinder(R)]