Département de chimie moléculaire

A rapid and sensitive electrochem. method for trypsin activity detn., using a gelatin coated glassy carbon electrode is reported. The hydrogel film creates a kinetic barrier for the transport of the electroactive hydroquinone to the electrode surface. The progressive trypsin catalyzed film digestion causes increase of its permeability, resulting in increase of the hydroquinone oxidn. current. Current response as a function of trypsin concn. within 10 min of incubation time was evaluated in the range of 0.1 to 1000 $μ$g mL-1. The limit of detection achieved in optimal conditions was found to be 0.01 $μ$g mL-1. [on SciFinder(R)]