Efficient Surface Patterning of Oligonucleotides Inside a Glass Capillary through Oxime Bond Formation.

The efficient surface patterning of oligonucleotides was accomplished onto the inner wall of fused-silica capillary tubes as well as on the surface of glass slides through oxime bond formation. The robustness of the method was demonstrated by achieving the surface immobilization of up to three different oligonucleotide sequences inside the same capillary tube. The method involves the prepn. of surfaces grafted with reactive aminooxy functionalities masked with the photocleavable protecting group, 2-(2-nitrophenyl) propyloxycarbonyl group (NPPOC). Briefly, NPPOC-aminooxy silane I was prepd. and used to silanize the glass surfaces. The NPPOC group was cleaved under brief irradn. to unmask the reactive aminooxy group on surfaces. These reactive aminooxy groups were allowed to react with aldehyde-contg. oligonucleotides to achieve an efficient surface immobilization. The advantage assocd. with the present approach is that it combines the high-coupling efficiency of oxime bond formation with the convenience assocd. with the use of photolabile groups. The present strategy thus offers an alternative approach for the immobilization of biomols. in the microchannels of "labs on a chip" devices. [on SciFinder(R)]

Références

Titre
Efficient Surface Patterning of Oligonucleotides Inside a Glass Capillary through Oxime Bond Formation.
Type de publication
Article de revue
Année de publication
2007
Revue
Bioconjug. Chem.
Volume
18
Pagination
671–676
ISSN
1043-1802
Soumis le 12 avril 2018