Efficient solid-phase chemical synthesis of 5'-triphosphates of DNA, RNA, and their analogues

A robust, reproducible, and scalable method for the solid-phase synthesis of 5'-triphosphates of DNA, RNA, and their chemically modified analogues using 5'-H-phosphonate intermediates is described. 5'-Triphosphates of oligonucleotides with varying lengths and sequences containing different 5'-terminal nucleotides, with and without internal sugar-backbone modifications, were efficiently prepared as crude products or further purified by HPLC.

Références

Titre
Efficient solid-phase chemical synthesis of 5'-triphosphates of DNA, RNA, and their analogues
Type de publication
Article de revue
Année de publication
2010
Revue
Organic Letters
Volume
12
Pagination
2190-3
Soumis le 5 juillet 2018