Efficient solid-phase chemical synthesis of 5'-triphosphates of DNA, RNA, and their analogues
A robust, reproducible, and scalable method for the solid-phase synthesis of 5'-triphosphates of DNA, RNA, and their chemically modified analogues using 5'-H-phosphonate intermediates is described. 5'-Triphosphates of oligonucleotides with varying lengths and sequences containing different 5'-terminal nucleotides, with and without internal sugar-backbone modifications, were efficiently prepared as crude products or further purified by HPLC.
Références
- Titre
- Efficient solid-phase chemical synthesis of 5'-triphosphates of DNA, RNA, and their analogues
- Type de publication
- Article de revue
- Année de publication
- 2010
- Auteurs
- Zlatev, I., Lavergne Thomas, Debart F., Vasseur J. J., Manoharan M., and Morvan F.
- Revue
- Organic Letters
- Volume
- 12
- Pagination
- 2190-3
Soumis le 5 juillet 2018