Direct electrochemistry and electrocatalysis of hemoglobin entrapped in composite matrix based on chitosan and CaCO3 nanoparticles.

The direct electron transfer between Hb and the underlying glassy carbon electrode (GCE) can be readily achieved via a high biocompatible composite system based on biopolymer chitosan (CHT) and inorg. CaCO3 nanoparticles (nano-CaCO3). Cyclic voltammetry of Hb-CHT/nano-CaCO3/GCE showed a pair of stable and quasi-reversible peaks for HbFe(III)/Fe(II) redox couple in pH 7.0 buffer. The electrochem. reaction of Hb immobilized in CHT/nano-CaCO3 composite matrix exhibited a surface-controlled process accompanied by electron and proton transfer. The electron transfer rate const. was estd. to be 1.8 s-1. This modified electrode showed a high thermal stability up to 60°. The apparent Michaelis-Menten const. was calcd. to be 7.5 × 10-4 M, indicating a high catalytic activity of the immobilized Hb toward H2O2. The interaction between Hb and this nano-hybrid material was also investigated using FT-IR and UV-vis spectroscopy, indicating that Hb retained its native structure in this hybrid matrix. [on SciFinder(R)]

Références

Titre
Direct electrochemistry and electrocatalysis of hemoglobin entrapped in composite matrix based on chitosan and CaCO3 nanoparticles.
Type de publication
Article de revue
Année de publication
2007
Revue
Electrochem. commun.
Volume
9
Pagination
529–534
ISSN
1388-2481
Soumis le 12 avril 2018