Development of amperometric biosensor for glucose based on a novel attractive enzyme immobilization matrix: Calcium carbonate nanoparticles.

Calcium carbonate nanoparticles (nano-CaCO3) may be a promising material for enzyme immobilization owing to their high biocompatibility, large sp. surface area and their aggregation properties. This attractive material was exploited for the mild immobilization of glucose oxidase (GOD) in order to develop glucose amperometric biosensor. The GOD/nano-CaCO3-based sensor exhibited a marked improvement in thermal stability compared to other glucose biosensors based on inorg. host matrixes. Amperometric detection of glucose was evaluated by holding the modified electrode at 0.60 V (vs. SCE) in order to oxidize the hydrogen peroxide generated by the enzymic reaction. The biosensor exhibited a rapid response (6 s), a low detection limit (0.1 $μ$M), a wide linear range of 0.001-12 mM, a high sensitivity (58.1 mA cm-2 M-1), as well as a good operational and storage stability. In addn., optimization of the biosensor construction, the effects of the applied potential as well as common interfering compds. on the amperometric response of the sensor were investigated and discussed herein. [on SciFinder(R)]

Références

Titre
Development of amperometric biosensor for glucose based on a novel attractive enzyme immobilization matrix: Calcium carbonate nanoparticles.
Type de publication
Article de revue
Année de publication
2007
Revue
Biosens. Bioelectron.
Volume
22
Pagination
1612–1617
ISSN
0956-5663
Soumis le 12 avril 2018