Département de chimie moléculaire

This paper reports on the use of electrochem. surface plasmon resonance (E-SPR) for the detection of carbohydrate-binding proteins. The generation of an SPR sensor specific to lectins Arachis hypogaea (PNA) and Maackia amurensis (MAA) is based on the electrochem. polymn. of oligosaccharide derivs. functionalized by pyrrole groups. The resulting thin conducting polymer films were characterized using E-SPR and at. force microscopy (AFM). The specific binding of PNA to polypyrrole-lactosyl and of MAA to polypyrrole-3'-sialyllactosyl films was investigated using SPR. The detection limit was 41 nM for PNA and 83 nM for MAA. Through Scatchard anal. and linear transformation of the SPR sensorgram data, assocn. (kass) and dissocn. rate consts. (kdiss) could be detd. [on SciFinder(R)]