Département de chimie moléculaire

The authors describe here a short and efficient synthetic route for incorporating terpyridine based metal complexes at the 3'-extremity of both single and bis-oligonucleotide (bis-ODN) stretches to form novel metal complex-ODN conjugates. All single stranded mono and bis-ODN tethered metal complexes and the resp. duplex ODNs were characterized by CD spectroscopy and UV-visible melting expts. Duplexes formed by these hybrid metal complex-DNA conjugates showed around 4-5° stabilization with respect to the unmodified duplexes. Also hybridization of two complementary bis-ODN tethered metal complexes at different ratios in soln. gave rise to a self-assembled nanometric linear network, which was characterized by nondenaturing gel electrophoresis and TEM studies. Thus, the authors' simple synthetic strategy would contribute to develop multimetallic 2-dimensional-DNA nanoarrays where the authors can place different metal complexes at regular intervals along the ODN stretches. [on SciFinder(R)]