Chemical Synthesis of Lesion-Containing Oligonucleotides for DNA Repair Studies

DNA is continuously exposed to various damaging conditions (oxidizing agents, UV, ionizing radiations, or endogenous species) that produce strand breaks, cross-links, or damaged nucleotides. In most cases, several kinds of DNA damages are simultaneously produced, often in low yield. Consequently, this hampered isolation and study of a well-defined damaged oligonucleotide (ODN). In order to get deeper insights into biological properties of a given lesion, chemistry enables preparation of large quantity of ODN containing a single and site-specifically located lesion. In addition, chemistry allows fine-tuning of lesions properties: influence of stereochemistry or of the neighboring nucleobase.

Références

Titre
Chemical Synthesis of Lesion-Containing Oligonucleotides for DNA Repair Studies
Type de publication
Book Chapter
Année de publication
2016
Auteurs
Lartia, Remy
Book Title
Modified Nucleic Acids in Biology and Medicine
Pagination
371–402
Éditeur
Springer International Publishing
Ville
Cham
ISBN
978-3-319-34175-0
Soumis le 18 mai 2018