Chemical Synthesis of Lesion-Containing Oligonucleotides for DNA Repair Studies
DNA is continuously exposed to various damaging conditions (oxidizing agents, UV, ionizing radiations, or endogenous species) that produce strand breaks, cross-links, or damaged nucleotides. In most cases, several kinds of DNA damages are simultaneously produced, often in low yield. Consequently, this hampered isolation and study of a well-defined damaged oligonucleotide (ODN). In order to get deeper insights into biological properties of a given lesion, chemistry enables preparation of large quantity of ODN containing a single and site-specifically located lesion. In addition, chemistry allows fine-tuning of lesions properties: influence of stereochemistry or of the neighboring nucleobase.
Références
- Titre
- Chemical Synthesis of Lesion-Containing Oligonucleotides for DNA Repair Studies
- Type de publication
- Book Chapter
- Année de publication
- 2016
- Auteurs
- Lartia, Remy
- Editor
- Jurga, Stefan, Deceased) Volker A. Erdmann, and Barciszewski Jan
- Book Title
- Modified Nucleic Acids in Biology and Medicine
- Pagination
- 371–402
- Éditeur
- Springer International Publishing
- Ville
- Cham
- ISBN
- 978-3-319-34175-0
Soumis le 18 mai 2018