Département de chimie moléculaire

The authors reported on the use of a novel attractive nanoscaled calcium carbonate (nano-CaCO3)-polyphenol oxidase (PPO) biocomposite to create a highly responsive phenol biosensor. The phenol sensor could be easily achieved by casting the biocomposite on the surface of glassy carbon electrode (GCE) via the crosslinking step by glutaraldehyde. The special three-dimensional structure, porous morphol., hydrophilic and biocompatible properties of the nano-CaCO3 matrix resulted in high enzyme loading, and the enzyme entrapped in this matrix retained its activity to a large extent. The proposed PPO/nano-CaCO3 exhibited dramatically developed anal. performance such as such as a broad detn. range (6 × 10-9-2 × 10-5 M), a short response time ({\textless}12 s), high sensitivity (474 mA M-1), subnanomolar detection limit (0.44 nM at a signal to noise ratio of 3) and good long-term stability (70{%} remained after 56 days). Effects of pH value, applied potential, temp. and electrode construction were studied and discussed. [on SciFinder(R)]