Département de chimie moléculaire

Biolayer Interferometry (BLI) and circular dichroism (CD) spectroscopies were used to investigate the interaction between previously reported i-motif DNA (i-DNA) ligands and folded or unfolded i-DNA in acidic (pH 5.5) and near-neutral (pH 6.5) conditions. We observed that although several ligands{,} in particular macrocyclic bis-acridine (BisA) and pyridostatin (PDS){,} showed good affinities for the telomeric i-motif forming sequence{,} none of the ligands displayed selective interactions with i-DNA structure nor was able to promote its formation.